A postdoctoral position is immediately available for a bioinformatician in the context of a long-term project that aims at understanding the molecular bases and therapeutic implications of cellular heterogeneity in human pancreatic cancer.
Pancreatic ductal adenocarcinoma (PDAC) is predicted to become the major cause of cancer cell deaths in the western world by 2030. It is nearly always an incurable disease, with a median survival time after diagnosis of four months. The causes of this extremely aggressive behavior are both the advanced stage of the disease at diagnosis and the peculiar biological properties of this tumor type, notably the co-occurrence within the same tumor of completely different and morphologically identifiable components: well-differentiated (low-grade) epithelial structures and nests of poorly differentiated (high-grade) quasi-mesenchymal tumor cells, whose coexistence reflects distinct underlying gene regulatory networks and transcriptional outputs.
This project is the continuation of a long-term research effort motivated by the assumption that the extreme heterogeneity of human PDACs is a critical determinant of the aggressive clinical behavior of these tumors. Our overall aim is to obtain a molecular characterization and mechanistic understanding of the transcriptional bases of cellular variability, eventually leading to the identification of novel mechanism-aware therapeutic options.
The project is highly integrative, making use of complementary cutting-edge technologies, including sequencing- and imaging-based spatial transcriptomics and epigenomics.
Your role will be to develop and apply computational methods for genomics research, large-scale data integration and analysis of sequencing data from complementary genomic technologies applied to primary human pancreatic cancer samples and to samples from experimental models developed in the lab.
Selected recent publications from the lab
1) FOXA2 controls the cis-regulatory networks of pancreatic cancer cells in a differentiation grade-specific manner (M. Milan…G. Natoli) EMBO Journal 15, 38(20):e102161 (2019).
2) Dissection of acute stimulus-induced nucleosome remodeling in mammalian cells (F. Comoglio… G. Natoli) Genes & Development 33: 1159-1174 (2019).
3) Cooptation of tandem DNA repeats for the maintenance of mesenchymal identity (C. Balestrieri…G. Natoli). Cell 173:1150-1164 (2018).
4) Opposing macrophage polarization programs show extensive epigenomic and transcriptional cross-talk (V. Piccolo …G. Natoli). Nature Immunology 18, 530-540. PMID 28288101 (2017).
5) High constitutive activity of a broad panel of housekeeping and tissue-specific cis-regulatory elements depends on a subset of ETS proteins (A. Curina A…G. Natoli) Genes & Development 31,399-412. PMID 28275002. (2017).
6) Dissection of transcriptional and cis-regulatory control of differentiation in human pancreatic cancer (G. Diaferia…G. Natoli). EMBO Journal 35, 596-617 PMID: 26769127 (2016).
7) Transcription of mammalian cis-regulatory elements is restrained by actively enforced early termination (L.M.I. Austenaa…G. Natoli). Molecular Cell 60, 460-474. PMID: 26593720 (2015).
8) A dual cis-regulatory code links IRF8 to constitutive and inducible gene expression in macrophages (A. Mancino…G. Natoli) Genes & Development 29, 394-408. PMID: 25637355. (2015).
9) Co-regulation of transcription factor binding and nucleosome occupancy through DNA features of mammalian enhancers (I. Barozzi…G. Natoli) Molecular Cell 54, 844-857. PMID: 24813947. (2014).
10) Latent enhancers activated by stimulation in differentiated cells (R. Ostuni…G. Natoli) Cell. 152: 157-71 (2013).