Position for one Postdoc is available in the laboratory of Dr. Luigi Nezi at European Institute of Oncology in Milan (Italy). Our Lab aims to develop innovative approaches in cancer prevention and therapy by manipulating the microbiome. We have reported the causality between diversity and composition of the gut microbiome at baseline and therapeutic outcome in patients of melanoma who were treated with immunotherapy (Science 2018). Moreover, our recent studies showed that compositional peculiarities of the tumor microenviroenement drive infiltrating T cells into a metabolic dysfunctional state (J Ex Med 2020). In the proposed project we will investigate (1) mechanisms involved in the interaction between the gut and tumor microbiome and their impact on anti-tumor immunoresponse and (2) how they influence tumor immunevasion and response to therapy. These goals will be achieved combining results from clinical and pre-clinical studies on different tumor models. The candidate will be part of an international team and have the possibility to actively interact with clinicians and worldwide-recognized experts in microbiome and immunology.
As part of your project you will:
- analyze gut and tumor microbiome, isolate and culture bacteria of therapeutic interest;
- perform immunophenotyping and functional studies on immune cell pupolations;
- participate in the development of ex vivo and in vivo models to study the interaction between microbiome and immunesystem;
- validate yoru findings in pre-clinical mouse models of melanoma, colorectal cancer and lung carcinoma.
Skills covered in this project:
- processing of human and mouse samples
- 16S rRNA and Metagenomic whole (shotgun) genome sequencing
- Culturing bacteria in aerobic and anaerobic conditions
- Immuophenotyping by polychromatic flow cytometry
- Immunological assays
- Multiplexed confocal imaging
- In vivo mouse experiments
- Ex vivo (organ-on-chip) and in vitro experiements
Overall, the results of this study will better inform the design of new strategies for cancer prevention and treatment. The candidate must hold a PhD degree in Microbiology, Biological Science or a similar subjects with a focus on Microbiology and Immunology. She/He should have documented experience on:
- DNA/RNA extraction and Next Generation Sequencing techniques
- Bacteria culturing
- Immunological assays (i.e. ELISA, immune cell isolation, in vitro activation assays)
- Mice handling and procedures for in vivo experiemnts
- Cell culturing
Scientific understanding of general research questions in molecular and cancer biology is essential. Ability to analyze omics data and code in R is a plus. Competitive salary based on professional experience.