One position for a Research Assistant on animal models immediately available in the laboratory of Dr. Luigi Nezi at European Institute of Oncology in Milan (Italy). Our Lab aims to develop innovative approaches in disease prevention and therapy by manipulating the microbiome. In particular, we have reported the causality between diversity and composition of the gut microbiome and therapeutic outcome in patients of melanoma who were treated with immunotherapy (Science 2018). Moreover, our recent studies showed that compositional peculiarities of the tumor microenviroenement (J. Ex. Med. 2020) and interactions with specific myeloid subpopulations (Nat. Comm. 2022) drive functional changes in infiltrating T cells, leading to a metabolic dysfunctional state or redirecting them toward a pro-tumoral phenotype, respectively.
In our lab we integrate metagenomic, metabolomic, functional assays and the use of pre-clinical models to decode the interplay between the microbiome and the host’s immunesystem during physiological and pathological conditions. The candidate will have the possibility to actively interact with scientists of different background embeddend in a highly dynamic and international environement. Our lab collaborate with worldwide-recognized experts in microbiome and immunological analisys. Overall, the results of our studies will better inform the design of new strategies for disease prevention and treatment.
Technical skills required for this position:
- The candidate must be qualified to perform functions A and D pursuant to Article 23 of Legislative Decree No. 26/14 "Attuazione della direttiva 2010/63/UE sulla protezione degli animali utilizzati a fini scientifici", being in possession of an appropriate level of education and training pursuant to Article 7, Paragraph 1 of Ministerial Decree. August 5, 2021 "Disciplina sulla formazione degli addetti ai compiti e alle funzioni di cui all’articolo 23, comma 2, del decreto legislativo n. 26/2014, in materia di protezione degli animali utilizzati a fini scientifici" or the requirements regulated by the transitional regulations set forth in Article 8 of the aforementioned Ministerial Decree.
- Cell culture of human and mouse primary cells and cell lines
- DNA/RNA purification and QC from different sources (i.e. tissues, blood, faeces, FFPE) and mouse genotyping.
The candidate should hold a Master degree in Molecular Science or a similar discipline. She/He should be highly organized, have a genuine predisposition for teamworking and good social attitude. Based on experience and workload, she/he could have the possibility to lead an independent scientific project, along with supporting other Lab members with in vivo experiements. Expertise in flow cytometry data acquisistion are plus. Scientific understanding of general research questions in molecular and cancer biology is required. Competitive salary based on experience.