We are seeking a highly motivated and enthusiastic predoctoral researcher with a background in molecular and cellular biology, and a strong interest in vaccine development, to join our team and contribute to this project.
RNA-based technologies have revolutionized the field of vaccinology, offering key advantages over traditional vaccine platforms. Despite these advances, the application of RNA-based vaccines in oncology remains challenging.
Our group has recently generated evidence that regulatory untranslated regions (UTRs) within RNA constructs can be strategically engineered to enhance antigen presentation via the MHC class I pathway.
Within this research framework, the laboratory is investigating how UTR sequences influence MHC class I antigen presentation and CD8⁺ T cell activation, with a particular focus on mRNA-based cancer vaccines.
The project will involve the screening and optimization of UTR elements using in-house methodologies. Experimental approaches will include DNA cloning, plasmid DNA (pDNA) amplification and purification, in vitro transcription (IVT) of RNA, RNA purification and characterization, and encapsulation into lipid nanoparticles, followed by quality control assessment. Functional evaluation will be performed both in vitro and in vivo using multiparametric flow cytometry, ex vivo functional assays, and T cell receptor (TCR) sequencing.
Skills and Qualifications
- Education: Master’s degree in a life sciences discipline, with demonstrated experience in molecular biology.
- Core Competencies: Proficiency in English (written and spoken), excellent organizational skills, and a strong ability to work both independently and collaboratively. Candidates should be highly motivated, intellectually curious, and proactive in driving their research projects.
- Technical Expertise: Prior hands-on experience with molecular biology techniques, including DNA cloning and in vitro transcription (IVT), is highly desirable. Experience in the manufacturing and characterization of nanoformulations using techniques such as Dynamic light scattering (DLS), ribogreen assay and TNS assay will be considered a plus.
Processing of Personal Data
The personal data, compulsorily provided, will be processed in compliance with Legislative Decree no. 101 of 10 August 2018, which adapts the Personal Data Protection Code (Legislative Decree no. 196 of 30 June 2003) to the provisions of Regulation (EU) 2016/679, and only for the fulfilments related to this procedure and for those consequent to the possible establishment of the working relationship, according to what is also provided for in the privacy policy, which must be accepted by each candidate at the time of sending his/her application.
Equal Opportunities
This notice is issued in compliance with equal opportunities between men and women for access to employment (L. 903/77) and economic treatment, pursuant to Legislative Decree no. 198/2006.
To apply, please send your CV, a short description of research interests through the appropriate link.
Posted on 10th April 2026