The Laboratory of Endocytosis, Signalling and Cancer directed by Prof. Pier Paolo Di Fiore at the Department of Experimental Oncology, European Institute of Oncology (IEO), Milan, Italy is recruiting a Postdoctoral researcher to investigate the role of the tumor suppressor NUMB in tumorigenesis, with a particular focus on breast cancer.
Loss of NUMB function is a frequent alteration in breast cancer, promoting disease aggressiveness and poor prognosis. At the molecular level, NUMB dysfunction leads to increased Notch oncogenic signalling and attenuation of the p53 tumour suppressor pathway. NUMB regulates p53 levels by binding to the ubiquitin-ligase Mdm2 and preventing it from triggering the proteasomal degradation of p53. Restoring NUMB function in breast cancer cells increases p53 levels and activity, sensitizing cells to chemotherapy. Thus, the NUMB:p53 axis represents a potential therapeutic target in breast cancer.
The successful candidate will focus on investigating the role of NUMB isoforms in breast cancer progression. Our research has shown that only specific NUMB isoforms interact with Mdm2 and regulate p53, and that their low levels correlate with poor prognosis in breast cancer. Therefore, deregulation of NUMB isoform expression appears to be a clinically relevant alteration in breast cancer patients. To further explore the distinct roles of these isoforms, we have identified isoform-specific interactors. The project will involve a detailed characterization of the most relevant interactors to determine their role in regulating the NUMB:p53 circuitry.
What we offer:
The successful candidate will join a highly motivated research group at the IEO in Milan, working under the supervision of Prof. Pier Paolo Di Fiore. The lab provides access to state-of-the-art facilities, cutting-edge expertise, and a collaborative research environment.
Required qualifications and skills:
- PhD in molecular and cellular biology or related fields.
- Excellent English communication skills, both written and verbal.
- Experience in cell biology, molecular biology and biochemistry techniques.
- Experience in mouse handling.
Desirable skills and experience:
- Experience in the production and purification of recombinant proteins.
- Experience in proteomics and phosphoproteomics techniques and analysis.
- Experience in light microscopy.
Salary will be commensurate with experience, qualifications, and skills.
Location:
The lab is located at the Department of Experimental Oncology, IEO, Via Adamello 16, 20141 Milan, Italy.
Interested candidates should send their application letter, CV, and full contact information of three referees by email to [email protected]
Posted on 25th June 2025