Imaging Development Unit
The Imaging Development Unit (IDU) has been created to develop high-end imaging applications requiring super resolution and high-content microscopy. It works on selected projects to create tools of general usefulness for cancer-research. The unit does not directly perform single experiments on demand but works with research groups by training researchers that actively participate to the development process and are educated to the use of the novel imaging technologies and generated solutions. IDU collaborates with companies in the microscopy field to test new instrumentation and develop new applications. Its activity focuses on overcoming the limitations of fluorescence microscopy to expand its use in basic and translational cancer research by producing new sample preparation protocols, acquisition pipelines and novel computational solutions. Computer-assisted, analysis-driven acquisition allows operating in a multi modal microscopy environment to reach 3 dimensional high- and super-spatial resolution by coupling widefield, confocal and single molecule localization microscopy to obtain a statistical sampling of thousands of events in the analysis of molecules, cells and tissues.
Most Relevant Publications
Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications
Automated multimodal fluorescence microscopy for hyperplex spatial-proteomics: Coupling microfluidic-based immunofluorescence to high resolution, high sensitivity, three-dimensional analysis of histological slides
Front Oncol, 2022
From Double-Strand Break Recognition to Cell-Cycle Checkpoint Activation: High Content and Resolution Image Cytometry Unmasks 53BP1 Multiple Roles in DNA Damage Response and p53 Action.
Int J Mol Sci, 2022
Novel Tools to Measure Single Molecules Colocalization in Fluorescence Nanoscopy by Image Cross Correlation Spectroscopy.
Nanomaterials (Basel), 2022