Imaging Unit

Simona Rodighiero

Unit Coordinator

*Email*	[email protected]
*Telephone*	+390294375026
*Location*	Building 1 Floor Ground Via Adamello 16, Milano

The Imaging Unit (IU) supports DEO scientific groups by offering cutting-edge optical imaging platforms and developing tailored imaging protocols and image analysis pipelines as per specific requirements.

More specifically, the IU activity include:

Conducting mandatory microscope training sessions to facilitate direct access to all IU microscopes
Providing image analysis support, including integration of machine learning into image analysis
Offering consulting services for sample preparation in optical microscopy experiments
Offering direct support and troubleshooting at the microscopes
Managing departmental imaging reagents
Teaching (SEMM PhD school and Master in Biomedical Omics of the University of Milan)

Additionally, IU staff actively participates in research collaborations with various research groups

Tools we use:

image analysis: Fiji/ImageJ, QuPath, ArivisVision 4D, NIS-Elements, Huygens, Python
data analysis: R, Python

Most Relevant Publications

Mazzarella L, Santoro F, Ravasio R, Fumagalli V, Massa PE, Rodighiero S, Gavilán E, Romanenghi M, Duso BA, Bonetti E, Manganaro L, Pallavi R, Trastulli D, Pallavicini I, Gentile C, Monzani S, Leonardi T, Pasqualato S, Buttinelli G, Di Martino A, Fedele G, Schiavoni I, Stefanelli P, Meroni G, de Francesco R, Steinkuhler C, Fossati G, Iannacone M, Minucci S, Pelicci PG

Inhibition of the lysine demethylase LSD1 modulates the balance between inflammatory and antiviral responses against coronaviruses.

Sci Signal, 2023
Russo M, Piccolo V, Polizzese D, Prosperini E, Borriero C, Polletti S, Bedin F, Marenda M, Michieletto D, Mandana GM, Rodighiero S, Cuomo A, Natoli G

Restrictor synergizes with Symplekin and PNUTS to terminate extragenic transcription.

Genes Dev, 2023
Nava Lauson CB, Tiberti S, Corsetto PA, Conte F, Tyagi P, Machwirth M, Ebert S, Loffreda A, Scheller L, Sheta D, Mokhtari Z, Peters T, Raman AT, Greco F, Rizzo AM, Beilhack A, Signore G, Tumino N, Vacca P, McDonnell LA, Raimondi A, Greenberg PD, Huppa JB, Cardaci S, Caruana I, Rodighiero S, Nezi L, Manzo T

Linoleic acid potentiates CD8+ T cell metabolic fitness and antitumor immunity.

Cell Metab, 2023

Technological Units

Imaging Unit

Simona Rodighiero

Most Relevant Publications

Inhibition of the lysine demethylase LSD1 modulates the balance between inflammatory and antiviral responses against coronaviruses.

Restrictor synergizes with Symplekin and PNUTS to terminate extragenic transcription.

Linoleic acid potentiates CD8+ T cell metabolic fitness and antitumor immunity.

Postdoc

Postdoctoral Imaging Analyst

Technician

Equipments

Common Imaging Reagents