The Biochemistry and Structural Biology Unit (BSU) at the European Institute of Oncology (IEO, Milan, Italy) is seeking a highly motivated research fellow for a position funded by the Regione Lombardia “Collabora e Innova” program, SAMET project.
The identified fellow will work on the biochemical and biophysical characterization of kinase fusion proteins in a wider project that integrates computational biology and biochemistry to determine the sensitivity or resistance to tyrosine kinase inhibitors (TKIs) in non-small cell lung cancer (NSCLC) patients.
Duration: 1 year, renewable
Salary: competitive salary depending on the candidate experience
Application deadline: 15th October 2025
Key responsibilities
- Cloning, expression, and purification of kinases and fusions in coli, insect cells and HEK cells
- Development of biochemical assays for kinase activity determination
- Biophysical characterization of the recombinant kinases
- Determination of the binding affinity with known ligands
- Contribution to experimental design, data analysis, and preparation of manuscripts and reports
Required qualifications and skills
- MSc degree in molecular biology, biochemistry, biophysics or structural biology (PhD not required)
- Previous experience in cloning, protein expression, and purification with FPLC systems
- Hands-on experience in protein biochemistry and enzyme characterization
- Willingness to learn a working methodology and biochemistry-related techniques
- Being proactive, fast thinking, precise, practical and well organized
Highly desirable skills
- Previous knowledge of protein biophysics techniques (e.g., mass photometry, ITC, BLI)
- Experience in kinase biology and enzymology
- Previous participation in structural biology projects (X-ray crystallography and/or cryo-EM) will be considered advantageous
Application Procedure
Applicants must submit their application through the appropriate link and should include:
- A detailed and updated CV
- A cover letter that describes your motivation to join the BSU
- Contact details of 2 referees
- Any other documentation considered useful for the selection
Informal enquiries can be addressed to Dr. Luigi Scietti ([email protected]). Applications through this channel will not be considered as formal. Please apply through the appropriate link.
Why IEO
The European Institute of Oncology (IEO) is a comprehensive Cancer Center, linking basic and translational research to clinical activities. The Department of Experimental Oncology (DEO) hosts about 250 researchers working in 26 independent groups. DEO is located in a scientific Campus together with the FIRC Institute of Molecular Oncology (IFOM) and the Italian Institute of Technology (IIT), and benefits from state-of-the-art Technological Units. The Biochemistry and Structural Biology Unit (BSU) can offer training, services, pipelines and state-of-the-art equipment for protein production and purification, biophysics, and access to structural biology platforms including cryo-EM and X-ray crystallography. IEO belongs to the EU-LIFE alliance for the promotion of science excellence in Europe. IEO is an equal opportunity employer committed to excellence through diversity.
PROCESSING OF PERSONAL DATA
The personal data, compulsorily provided, will be processed in compliance with Legislative Decree no. 101 of 10 August 2018, which adapts the Personal Data Protection Code (Legislative Decree no. 196 of 30 June 2003) to the provisions of Regulation (EU) 2016/679, and only for the fulfilments related to this procedure and for those consequent to the possible establishment of the working relationship, according to what is also provided for in the privacy policy, which must be accepted by each candidate at the time of sending his/her application.
EQUAL OPPORTUNITIES
This notice is issued in compliance with equal opportunities between men and women for access to employment and economic treatment, pursuant to Legislative Decree no. 198/2006.3
Posted on 23rd September 2025