The Department of Experimental Oncology (DEO) of the European Institute of Oncology (IEO) in Milan is recruiting a Research Assistant in the field of Precision Medicine - Next-Generation Sequencing (NGS). The position will be under the supervision of dr Luca Mazzarella, MD PhD, in the Laboratory of Translational Oncology, dedicated to the study of cancer using multidisciplinary approaches.
The successful candidate will conduct high-quality sequencing analyses for biomedical research and genomic medicine, and assist in CRISPR-based functional genomics experiments
General responsibilities include handling and preparation of bio-samples; various molecular biology assays such as library construction and quality control for next generation sequencing. The position includes bench work involving, e.g., bio-specimen processing, nucleic acid extraction, construction of sequencing libraries, sample quality control and sequencing, DNA cloning and tissue culture.
After appropriate training the successful candidate is expected to:
-independently handle advanced genomics instrumentation including e.g., Illumina/Thermofisher/Nanopore next generation sequencing platforms and a broad spectrum of quality control instruments
-be able to independently perform tissue culture-based experiments and DNA cloning
- B.Sc., M.Sc., molecular biology or a related field
- ‘Hands-on’ experience and knowledge in genomics and molecular biology, such as DNA/RNA extraction, PCR, qPCR.
- Demonstrated ability to work independently in performance of laboratory processes such as experimentation, quality assessment, analysis, and documentation of results
- Good communication skills. Must be able to actively elicit feedback from staff.
- Ability and desire to work with a collaborative, fast‐paced project team in an entrepreneurial work environment
- Experience in DNA/RNA library preparation workflows, including, amplicon, hybrid capture, and RNAseq as well as operation of Illumina/Nanopore/ Thermofisher NGS sequencing.
- Experience with tissue culture, DNA cloning, CRISPR-Cas9, Western Blotting